LOCUS       sequence-117644-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively express mitochondrial localized PhyB in Saccharomyces
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Jost AP, Weiner OD
  TITLE     Probing Yeast Polarity with Acute, Reversible, Optogenetic 
            Inhibition of Protein Function.
  JOURNAL   ACS Synth Biol. 2015 Jun 2.
  PUBMED    26035630
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Jun 2."
COMMENT     Sequence Label: PhyB-mCherry-Tom7
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..39
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        327..734
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 catgattacg ccaagctcgg aattaaccct cactaaaggg aacaaaagct gggccggcca
       61 tttaaatgtt taaacggcca cgctggccga ggagaacttc tagtatatct acatacctaa
      121 tattattgcc ttattaaaaa tggaatccca acgattacat caaaatccac attctcttca
      181 aaatcaattg tcctgtactt ccttgttcat gtgtgttcaa aaacgttata tttataggat
      241 aattatactc tatttctcaa caagtaattg gttgtttggc cgagcggtct aaggcgcctg
      301 attcaagaaa tatcttgacc gcagttaact gtgggaatac tcaggtatcg taagatgcaa
      361 gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg
      421 cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc
      481 tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg
      541 aaccggcttt tcatatagaa tagagaagcg ttcatgacta aatgcttgca tcacaatact
      601 tgaagttgac aatattattt aaggacctat tgttttttcc aataggtggt tagcaatcgt
      661 cttactttct aacttttctt accttttaca tttcagcaat atatatatat atatttcaag
      721 gatataccat tctaatgtct gcccctaagt taccaataga tagcggttta atataaggtt
      781 tcaacaagaa atattttgtg aaaatcaaaa gttaatagtt tcactgtcga aaatatgaaa
      841 gtttaagttt tcagttcatc cccattgtag ttatcaaatt tctgtgtttc ccgtatatgt
      901 gtgctctgta ttcatcgcta tcttgaaact ggttatgatc gttgcgcatg tgacctgtat
      961 cgctcaaaag agcc
//
