LOCUS       sequence-117722-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Macrolide biosensor. Medium copy plasmid that expresses GFP 
            proportional to macrolide concentration. Compatible with all other 
            pJKR plasmids..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-O-mphR
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             487..495
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
       61 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
      121 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
      181 cgccactcca ccggcggcat ggacgagctg tacaagtaac taatgagtga gctaacttac
      241 attaattgcg ttgcgcctta attaacggca ctcctcagca aatataatga ccctcttgat
      301 aacccaagag ggcatttttt aatgcccatg gcgtttacca cagctaacac cacgtcgtcc
      361 ctatctgctg ccctaggtct atgagtggtt gctggataac tttacgggca tgcataaggc
      421 tcgtaggcta tattcaggga gaccacaacg gtttccctct acaaataatt ttgtttaact
      481 ttgaaataag gaggtaatac aaatgccgcg tccgaaactg aaatctgacg acgaagttct
      541 ggaagcggcg accgttgttc tgaaacgttg cggtccgatc gaattcaccc tgtctggtgt
      601 tgcgaaagaa gttggtctgt ctcgtgcggc gctgatccag cgtttcacca accgtgacac
      661 cctgctggtt cgtatgatgg aacgtggtgt tgaacaggtt cgtcactacc tgaacgcgat
      721 cccgatcggt gcgggtccgc agggtctgtg ggaattccc
//
