LOCUS       sequence-117723-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human JMJD3 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Agger K, Cloos PA, Christensen J, Pasini D, Rose S, Rappsilber J, 
            Issaeva I, Canaani E, Salcini AE, Helin K
  TITLE     UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene 
            regulation and development.
  JOURNAL   Nature. 2007 Oct 11. 449(7163):731-4.
  PUBMED    17713478
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Oct 11. 449(7163):731-4."
COMMENT     Sequence Label: pCMV-HA-JMJD3
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             162..188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    201..225
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(661..672)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtttgagatg aggataaaat actctgagtc caaaccgggt ccctctgcta accatgttca
       61 tgccttcttc tctttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca
      121 ttttggcaaa gaattcctcg acggatcaat tcaccatggc atacccatac gatgttccag
      181 attacgctag cggatcatca acaagtttgt acaaaaaagc aggctcttta aaggaaccaa
      241 ttcagtcgac tggatccggt acctggatgc atcgggcagt ggatcctcca ggggcccgcg
      301 ctgcacggga agcctttgcc cttgggggcc tgagctgtgc tggggcctgg agctcctgcc
      361 cgcctcatcc ccctcctcgt agcgcatggc tgcctggagg cagatgctca gccagcattg
      421 ggcagccccc gcttcctgct cccctacccc cttcacatgg cagtagttct gggcacccca
      481 gcaaaccata ttatgctcca ggggcgccca ctccaagacc cctccatggg aagctggaat
      541 ccctacatgg ctgtgtgcag gcattgctcc gggagccagc ccagccaggg ctttgggaac
      601 agcttgggca actgtacgag tcagagcacg atagtgagga ggccacacgc tgctaccaca
      661 gcgcccttcg atacggagga agcttcgctg agctggggcc ccgcattggc cgactgcagc
      721 aggcccagct ctggaacttt catactgg
//
