LOCUS       sequence-117741-         465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans 
            K08F4.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP334-2
FEATURES             Location/Qualifiers
     source          1..465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(249..324)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
       61 ggctggctta actatgcggc atcagagcag attgcactga gagtgcacca tatgcggtgt
      121 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
      181 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagaaattt
      241 cacaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta
      301 acttgctatt tctagctcta aaacctgcgc ataccacctc gtgcaagaca tctcgcaata
      361 ggaggtgttt atataggggc aacacaagag gcggcagaca tctcgtgagt ttgacagagt
      421 agtataaaga gtgtgtgtga gagggtgtag gcatttcata acata
//
