LOCUS       sequence-117742-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay vector, assessing regulation of 300-400 nucleotide 
            CA repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psicheck_300_400_CA_repeat
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          118..250
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     233..252
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        295..313
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      317..326
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgattct
       61 tctgacacaa cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggta
      121 agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag
      181 agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt
      241 ctctccacag gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac
      301 gactcactat aggctagcca ccatggcttc caaggtgtac gaccccgagc aacgcaaacg
      361 catgatcact gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt
      421 catcaactac tatgattccg agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa
      481 cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac atcgagcccg tggctagatg
      541 catcatccct gatctgatcg gaatgggtaa gtccggcaag agcgggaatg gctcatatcg
      601 cctcctggat cactacaagt acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa
      661 aatcatcttt gtgggccacg actggggggc ttgtctggcc tttcactact cctacgagca
      721 ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga tcgag
//
