LOCUS       sequence-117744-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay vector, assessing regulation of 200-300 nucleotide 
            CA repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psicheck_200_300_CA_repeat
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          127..259
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     242..261
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        304..322
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      326..335
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
       61 cttgattctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc gtgaggcact
      121 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
      181 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
      241 tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt
      301 acttaatacg actcactata ggctagccac catggcttcc aaggtgtacg accccgagca
      361 acgcaaacgc atgatcactg ggcctcagtg gtgggctcgc tgcaagcaaa tgaacgtgct
      421 ggactccttc atcaactact atgattccga gaagcacgcc gagaacgccg tgatttttct
      481 gcatggtaac gctgcctcca gctacctgtg gaggcacgtc gtgcctcaca tcgagcccgt
      541 ggctagatgc atcatccctg atctgatcgg aatgggtaag tccggcaaga gcgggaatgg
      601 ctcatatcgc ctcctggatc actacaagta cctcaccgct tggttcgagc tgctgaacct
      661 tccaaagaaa atcatctttg tggg
//
