LOCUS       sequence-117746-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay vector, assessing regulation of SRM1 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psicheck_SRM1_3UTR
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          109..241
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     224..243
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        286..304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      308..317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgattc ttctgacaca
       61 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      121 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      181 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      241 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
      301 taggctagcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac
      361 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta
      421 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc
      481 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc
      541 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga
      601 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt
      661 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc acc
//
