LOCUS       sequence-117749-         430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay vector, assessing regulation of ETF1 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 430)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psicheck_ETF1_3UTR
FEATURES             Location/Qualifiers
     source          1..430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          27..159
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     142..161
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        205..223
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      227..236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagttggtcg tcgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag
       61 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
      121 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt
      181 acagctctta aaggctagag tacttaatac gactcactat aggctagcca ccatggcttc
      241 caaggtgtac gaccccgagc aacgcaaacg catgatcact gggcctcagt ggtgggctcg
      301 ctgcaagcaa atgaacgtgc tggactcctt catcaactac tatgattccg agaagcacgc
      361 cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt
      421 cgtgcctcac
//
