LOCUS       sequence-117753-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans 
            K08F4.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP334-6
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(9..332)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(347..373)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(374..394)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agttgtgaga cttttttctt ggcggcacaa taaagtttcc tctttttgtt gggtcactca
       61 acgaacacaa tacaaatatt tgagcgctcg catttttctc aacctttcgg gtttaaagca
      121 cttcaaaatg gaattgaaga gagattgtga aagtacatca gacagaatca cattttgata
      181 gcattcactt cactcagatg caaacttttc agtgactata aaaagtcata aaataataac
      241 attaaaaaag caaataaatt agcgagagaa ttttttgaca aaaagaaaga agagtgatag
      301 agaagaaggg aatgcttgaa aggattttgc atttatcact agtttaggcg tagtctggga
      361 cgtcgtatgg gtagacctta cgcttcttct ttggtggtcc ggatcctccg tctcctccga
      421 gttgggagag gtcgatacgg gtctcgtaga gtccggtgat ggattggtgg atgagggtgg
      481 cgtcgaggac ctccttggtg gaggtgtaac gcttacggtc gatggtggtg tcgaagtact
      541 tgaaggcggc tggggctccg aggttggtga gggtgaagag gtggatgatg ttctcggctt
      601 gctcacggat tggcttgtca cggtgcttgt tgtaggcgga gaggaccttg tcgaggttgg
      661 cgtcggcgag gatgacacgc ttggagaact cggagatttg ctcgatgatc tcgtcgaggt
      721 agtgcttgtg ttgctcgacg aagagttgct tttgctcgtt gtcctct
//
