LOCUS       sequence-117755-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay vector, assessing regulation of UBE2V1 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psicheck_UBE2V1_3UTR
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          124..256
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     239..258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        301..319
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      323..332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
       61 gattcttctg acacaacagt ctcgaactta agctgcagaa gttggtcgtg aggcactggg
      121 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
      181 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
      241 gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg ctagagtact
      301 taatacgact cactataggc tagccaccat ggcttccaag gtgtacgacc ccgagcaacg
      361 caaacgcatg atcactgggc ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga
      421 ctccttcatc aactactatg attccgagaa gcacgccgag aacgccgtga tttttctgca
      481 tggtaacgct gcctccagct acctgtggag gcacgtcgtg cctcacatcg agcccgtggc
      541 tagatgcatc atccctgatc tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc
      601 atatcgcctc ctggatcact acaagtacct caccgcttgg ttcgagctgc tgaaccttcc
      661 aaagaaaatc atctttgtgg gccacgactg gggggcttgt ctggcctttc actactccta
      721 cgagca
//
