LOCUS       sequence-117767-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay vector, assessing regulation of NDUFA6 3' UTR, with
            two mutated tandem CAC motifs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psicheck_NDUFA6_3UTR_mut
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          112..244
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     227..246
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        289..307
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      311..320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttcttctgac
       61 acaacagtct cgaacttaag ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc
      121 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga
      181 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc
      241 acaggtgtcc actcccagtt caattacagc tcttaaggct agagtactta atacgactca
      301 ctataggcta gccaccatgg cttccaaggt gtacgacccc gagcaacgca aacgcatgat
      361 cactgggcct cagtggtggg ctcgctgcaa gcaaatgaac gtgctggact ccttcatcaa
      421 ctactatgat tccgagaagc acgccgagaa cgccgtgatt tttctgcatg gtaacgctgc
      481 ctccagctac ctgtggaggc acgtcgtgcc tcacatcgag cccgtggcta gatgcatcat
      541 ccctgatctg atcggaatgg gtaagtccgg caagagcggg aatggctcat atcgcctcct
      601 ggatcactac aagtacctca ccgcttggtt cgagctgctg aaccttccaa agaaaatcat
      661 ct
//
