LOCUS       sequence-117775-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans 
            swan-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: APCSD 54
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..604
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 gttggcacct ttggtctttt attgtcaact tccattggtt cttccattgt ttctgttaaa
       61 ttaatgaatt tttcataaaa taaagacatt atacaatata aaaatgaaga atttattgaa
      121 aataaactgc cagagagaaa aagtatgcaa cactcccgcc gagagtgttt gaaatggtgt
      181 acggtacatt ttcgtgctag gagttagatg tgcaggcagc aacgagaggg ggagagattt
      241 ttttgggcct tgtgaaatta acgtgagttt tctggtcatc tgactaatca tgttggtttt
      301 ttgttggttt attttgtttt tatctttgtt tttatccaga ttaggaaatt taaattttat
      361 gaatttataa tgaggtcaaa cattcagtcc cagcgttttt cctgttctca ctgtttagtc
      421 gaatttttat tttaggcttt caacaaatgt tctaactgtc ttatttgtga cctcactttt
      481 tatatttttt taatttttaa aaatattaga agtttctagg ataatttttt cgacttttat
      541 tctctctacc gtccgcactc ttcttacttt taaattaaat tgtttttttt tcagttggga
      601 aacactttgc tctagaaaaa atggacaaaa aatacagcat cggccttgac atcggaacga
      661 actccgtcgg atgggccgtc atcaccgacg agtacaaggt
//
