LOCUS       sequence-117776-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-activated AAV expression of humanized ChR2 with H134R mutation 
            fused to mCherry for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  TITLE     Cre-activated AAV expression plasmids
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-EF1a-double
            floxed-hChR2(H134R)-mCherry-W...
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(22..55)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(106..139)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(429..448)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     698..716
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tcctctagag tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga
       61 atggtagctg gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc
      121 atacattata cgaagttatg gcgcgcccta ttacttgtac agctcgtcca tgccgccggt
      181 ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga
      241 ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc
      301 cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg
      361 cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca
      421 gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt
      481 caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc
      541 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa
      601 gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg
      661 ggacaggatg tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt
      721 ctggg
//
