LOCUS       sequence-117777-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-activated AAV expression of humanized ChR2 with H134R mutation 
            fused to mCherry for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  TITLE     Cre-activated AAV expression plasmids
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-EF1a-double
            floxed-hChR2(H134R)-mCherry-W...
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(131..164)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      169..178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag
       61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg
      121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatgctagc caccatggac
      181 tatggcggcg ctttgtctgc cgtcggacgc gaacttttgt tcgttactaa tcctgtggtg
      241 gtgaacgggt ccgtcctggt ccctgaggat caatgttact gtgccggatg gattgaatct
      301 cgcggcacga acggcgctca gaccgcgtca aatgtcctgc agtggcttgc agcaggattc
      361 agcattttgc tgctgatgtt ctatgcctac caaacctgga aatctacatg cggctgggag
      421 gagatctatg tgtgcgccat tgaaatggtt aaggtgattc tcgagttctt ttttgagttt
      481 aagaatccct ctatgctcta ccttgccaca ggacaccggg tgcagtggct gcgctatgca
      541 gagtggctgc tcacttgtcc tgtcatcctt atccgcctga gcaacctcac cggcctgagc
      601 aacgactaca gcaggagaac catgggactc cttgtctcag acatcgggac tatcgtgtgg
      661 ggggctacca gcgccatggc aaccggctat gttaaagtca tcttcttttg tcttggattg
      721 tgctatggcg cgaacacatt ttttcacgcc gccaaagcat atatcgaggg ttatcatact
      781 gtg
//
