LOCUS       sequence-117788-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal YFP fragment was fused to Ggamma-1. When 
            co-expressed with an amino-terminal YFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot
            CH
  TITLE     Visualization of G protein betagamma dimers using bimolecular 
            fluorescence complementation demonstrates roles for both beta and 
            gamma in subcellular targeting.
  JOURNAL   J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10.
  PUBMED    15136579
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-07-16"; volume: "279(29)"; pages: "30279-86. 
            Epub 2004 May 10"
COMMENT     Sequence Label: YFP(159-238)-gamma-1 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(303..324)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      506..515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(546..565)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(547..565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(610..813)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(639..659)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcgagcgg ccgccagtgt gatagatctt tatgaaatca cacagcctcc tttgagctcc
       61 ttgaagggat ttttgtcctc tgggataccc tttactaatg gatcttcccc ggatctttct
      121 tcaacataat ccctgaattc ttcacaacat ttggacacca gcattctttc cagcgtcact
      181 tctttcttga gctggtcgac ttccatcttc aatttgtcct tttctgtcag gtcctcaata
      241 ttgatcactg gcatggatct cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
      301 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
      361 gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      421 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      481 aagttcacct tgatgccgtt cttgcccatg gtggcggatc cgagctcggt accaagcttg
      541 ggtctcccta tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt
      601 tagccagaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
      661 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
      721 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
      781 acgcccattg atgtactgcc aaaaccgcat caccatggt
//
