LOCUS       sequence-117791-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal YFP fragment was fused to Ggamma-2. When 
            co-expressed with an amino-terminal YFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot
            CH
  TITLE     Visualization of G protein betagamma dimers using bimolecular 
            fluorescence complementation demonstrates roles for both beta and 
            gamma in subcellular targeting.
  JOURNAL   J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10.
  PUBMED    15136579
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-07-16"; volume: "279(29)"; pages: "30279-86. 
            Epub 2004 May 10"
COMMENT     Sequence Label: YFP(159-238)-gamma-2 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(279..300)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      482..491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(522..541)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(523..541)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(586..685)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(615..635)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agtgtgatag atctttaaag gatagcacag aaaaacttct tctccctaaa tgggttttct
       61 gaagccggaa caggtgtcag gaggggatct tccttggcat gcgcttcaca gtaggccatc
      121 aaatctgcag ctgccttgga cacctttatc ctatcgatgt tggcttccat cttcagctgt
      181 tctaccagtt tcctggcttg tgctatgctg gcggtgttgt tgctggccat ggatctcttg
      241 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      301 tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag gtagtggttg
      361 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      421 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttg
      481 cccatggtgg cggatccgag ctcggtacca agcttgggtc tccctatagt gagtcgtatt
      541 aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata
      601 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
      661 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caat
//
