LOCUS       sequence-117797-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used in the triple transfection method of AAV 
            vector production. It supplies the replication proteins from AAV2 
            and the engineered shh10 capsid protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Klimczak RR, Koerber JT, Dalkara D, Flannery JG, Schaffer DV
  TITLE     A novel adeno-associated viral variant for efficient and selective 
            intravitreal transduction of rat Muller cells.
  JOURNAL   PLoS One. 2009 Oct 14;4(10):e7467. doi: 
            10.1371/journal.pone.0007467.
  PUBMED    19826483
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-10-14"; volume: "4(10):e7467. doi"; pages: " 
            10.1371/journal.pone.0007467"
COMMENT     Sequence Label: shh10
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             747..758
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atcctctcat cgaccagtac ctgtattacc tgaacagaac tcaggatcag tccggaagtg
       61 cccaaaacaa ggacttgctg tttagccggg ggtctccagc tggcatgtct gttcagccca
      121 aaaactggct acctggaccc tgttaccggc agcagcgcgt ttctaaaaca aaaacagaca
      181 acaacaacag caactttacc tggactggtg cttcaaaata taaccttaat gggcgtgaat
      241 ctataatcaa ccctggcact gctatggcct cacacaaaga cgacaaaaac aagttctttc
      301 ccatgagcgg tgtcatgatt tttggaaagg agagcgccgg agcttcaaac actgcattgg
      361 acaatgtcat gatcacagac gaagaggaaa tcaaagccac taaccccgtg gccaccgaaa
      421 gatttgggac tgtggcagtc aatctccaga gcagcagcac agaccctgcg accggagatg
      481 tgcatgttat gggagcctta cctggaatgg tgtggcaaga cagagacgta tacctgcagg
      541 gtcctatttg ggccaaaatt cctcacacgg atggacactt tcacccgtct cctctcatgg
      601 gcggctttgg acttaagcac ccgcctcctc agatcctcat caaaaacacg cctgttcctg
      661 cgaatcctcc ggcagagttt tcggctacaa agtttgcttc attcatcacc cagtattcca
      721 caggacaagt gagcgtggag attgaatggg agctgcagaa agaaaacagc aaacgctgga
      781 atcccgaagt gcagtataca tctaactatg caaaatctgc caacgttgat ttcactgtgg
      841 acaacaatgg actttatact gagcctcgcc ccattggcac ccgttacctc acccgtcccc
      901 tgtaattgcg gccgcttgtt aatcaataaa
//
