LOCUS       sequence-117798-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used in the triple transfection method of AAV 
            vector production. It supplies the replication proteins from AAV2 
            and the engineered shh10 capsid protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Klimczak RR, Koerber JT, Dalkara D, Flannery JG, Schaffer DV
  TITLE     A novel adeno-associated viral variant for efficient and selective 
            intravitreal transduction of rat Muller cells.
  JOURNAL   PLoS One. 2009 Oct 14;4(10):e7467. doi: 
            10.1371/journal.pone.0007467.
  PUBMED    19826483
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-10-14"; volume: "4(10):e7467. doi"; pages: " 
            10.1371/journal.pone.0007467"
COMMENT     Sequence Label: shh10
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     122..141
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(818..839)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
       61 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
      121 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
      181 tgcgtaagga gaaaataccg catcaggcga ttccaacatc caataaatca tacaggcaag
      241 gcaaagaatt agcaaaatta agcaataaag cctcagagca taaagctaaa tcggttgtac
      301 caaaaacatt atgaccctgt aatacttttg cgggagaagc ctttatttca acgcaaggat
      361 aaaaattttt agaaccctca tatattttaa atgcaatgcc tgagtaatgt gtaggtaaag
      421 attcaaacgg gtgagaaagg ccggagacag tcaaatcacc atcaatatga tattcaaccg
      481 ttctagctga taaattcatg ccggagaggg tagctatttt tgagaggtct ctacaaaggc
      541 tatcaggtca ttgcctgaga gtctggagca aacaagagaa tcgatgaacg gtaatcgtaa
      601 aactagcatg tcaatcatat gtaccccggt tgataatcag aaaagcccca aaaacaggaa
      661 gattgtataa gcaaatattt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
      721 tttttgttaa atcagctcat tttttaacca atatgccgaa atcggcaaaa tcccttataa
      781 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
      841 attaaagaac gtggac
//
