LOCUS       sequence-117800-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used in the triple transfection method of AAV 
            vector production. It supplies the replication proteins from AAV2 
            and the 7M8 capsid protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Dalkara D, Byrne LC, Klimczak RR, Visel M, Yin L, Merigan WH, 
            Flannery JG, Schaffer DV
  TITLE     In vivo-directed evolution of a new adeno-associated virus for 
            therapeutic outer retinal gene delivery from the vitreous.
  JOURNAL   Sci Transl Med. 2013 Jun 12;5(189):189ra76. doi: 
            10.1126/scitranslmed.3005708.
  PUBMED    23761039
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2013-06-12"; volume: "5(189):189ra76. doi"; pages: " 
            10.1126/scitranslmed.3005708"
COMMENT     Sequence Label: 7M8
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             777..788
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atcctctcat cgaccagtac ctgtattact tgagcagaac aaacactcca agtggaacca
       61 ccacgcagtc aaggcttcag ttttctcagg ccggagcgag tgacattcgg gaccagtcta
      121 ggaactggct tcctggaccc tgttaccgcc agcagcgagt atcaaagaca tctgcggata
      181 acaacaacag tgaatactcg tggactggag ctaccaagta ccacctcaat ggcagagact
      241 ctctggtgaa tccgggcccg gccatggcaa gccacaagga cgatgaagaa aagttttttc
      301 ctcagagcgg ggttctcatc tttgggaagc aaggctcaga gaaaacaaat gtggacattg
      361 aaaaggtcat gattacagac gaagaggaaa tcaggacaac caatcccgtg gctacggagc
      421 agtatggttc tgtatctacc aacctccaga gaggcaacct agcactcggc gaaacaacaa
      481 gacctgctag gcaagcagct accgcagatg tcaacacaca aggcgttctt ccaggcatgg
      541 tctggcagga cagagatgtg taccttcagg ggcccatctg ggcaaagatt ccacacacgg
      601 acggacattt tcacccctct cccctcatgg gtggattcgg acttaaacac cctcctcccc
      661 agattctcat caagaacacc ccggtacctg cgaatccttc gaccaccttc agtgcggcaa
      721 agtttgcttc cttcatcaca cagtactcca cgggacaggt cagcgtggag atcgagtggg
      781 agctgcagaa ggaaaacagc aaacgctgga atcccgaaat tcagtacact tccaactaca
      841 acaagtctat taatgtggac tttactgtgg acactaatgg cgtgtattca gagcctcgcc
      901 ccattggcac cagatacctg actcg
//
