LOCUS       sequence-117802-         387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp-gfpmut3
FEATURES             Location/Qualifiers
     source          1..387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      122..208
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(340..387)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gttttttatt ggtgagaatc caagctagac tgcgatgagt ggcagggcgg ggcgtaattt
       61 ttttaaggca gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca
      121 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      181 ggtgaacgct ctcctgagta ggacaaatcc gccgctagga gcttgcggcc cgccttcgcg
      241 aggtaccggg cccaagcttc tcgaggaatt cctgcagccc gggggatcca ctagtgagct
      301 catgcatgat cgagctcgaa ttagcttcaa aagcgctctg aagttcctat actttctaga
      361 gaataggaac ttcggaatag gaacttc
//
