LOCUS       sequence-117804-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TNF reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: TNF-SBP-EGFP_puromycin
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatcgatat ctgcggccta gctagcggga
      121 ggcgcgccat gtctaccgag tctatgatta gggacgtgga actggctgag gaggcactgc
      181 ccaaaaaaac cggcggacca cagggctcta ggagatgtct gtttctgtct ctgttctctt
      241 ttctcatcgt ggctggcgct acaacactct tctgtctgct ccatttcggc gtgattggac
      301 cacagcgaga ggaatttccc cgggatctgt cactcatttc accactggca caggctgtcc
      361 gatcttcatc tcggactcca tccgacaaac ctgtcgccca tgtcgtcgcc aacccacagg
      421 ccgagggcca gctccagtgg ctcaatagga gggcaaacgc tctgctcgcc aatggcgtgg
      481 aactccggga taaccagctc gtcgtgccta gtgagggact gtacctcatc tactcccagg
      541 tgctgtttaa gggccaggga tgtccttcta cacatgtgct gctcacacac acaatttcac
      601 ggatcgccgt gtcttaccag actaaagtca atctgctctc tgccatcaaa tccccatgtc
      661 agcgggaaac acctgagggc gctgaggcta aaccttggta cgaacccatc tacctcggag
      721 gcgtgttcca gctggagaag ggcgatagac tgagtgctga gatcaatcga cccgactacc
      781 tcgactttgc cgaatctggc caggtctact ttggcatcat tgctct
//
