LOCUS       sequence-117805-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TNF reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: TNF-SBP-EGFP_puromycin
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..132)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 gctcaccata cctgcaggtg gttcacgttg accttgtggg tgatgctcca gtcgtgcacg
       61 aagttgttca agttccccag ccagtccttc aacaacgtgt ccacctcgcc aaccagtggt
      121 cttctcgtcc atggaattca aaggcctagg tgcgccatga gctcggccct cgaggcccag
      181 agcaatgatg ccaaagtaga cctggccaga ttcggcaaag tcgaggtagt cgggtcgatt
      241 gatctcagca ctcagtctat cgcccttctc cagctggaac acgcctccga ggtagatggg
      301 ttcgtaccaa ggtttagcct cagcgccctc aggtgtttcc cgctgacatg gggatttgat
      361 ggcagagagc agattgactt tagtctggta agacacggcg atccgtgaaa ttgtgtgtgt
      421 gagcagcaca tgtgtagaag gacatccctg gcccttaaac agcacctggg agtagatgag
      481 gtacagtccc tcactaggca cgacgagctg gttatcccgg agttccacgc cattggcgag
      541 cagagcgttt gccctcctat tgagccactg gagctggccc tcggcctgtg ggttggcgac
      601 gacatgggcg acaggtttgt cggatggagt ccgagatgaa gatcggacag cctgtgccag
      661 tggtgaaatg agtgacagat cccggggaaa ttcctctcgc tgtggtccaa tcacgccg
//
