LOCUS       sequence-117817-        9852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9852)
  TITLE     Guillaume Filion Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pC-sleeping beauty
FEATURES             Location/Qualifiers
     source          1..9852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    42..471
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     CDS             complement(564..1586)
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     primer_bind     complement(1596..1615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1597..1615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1660..2108)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(1692..1713)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     gene            2127..6263
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2614..2685,3080..3353,3428..4082,4144..4459,4680..4811,
                     4882..5496)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(6274..6828,6829..6859)
                     /label=P element 5' end
     rep_origin      complement(7094..7682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7174..7193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7853..8644,8645..8713))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8476..8495
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8714..8818)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8886..8904
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8942..8964)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9064..9083
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9620..9650,9651..9852)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttggct gcaggtcgac ggatccccgt taattcgagc tctcctgacc gtccatcgca
       61 ataaaatgag ccattatgag atcgaaaggg tctacgaaaa tccgttcgga aaaaatcaga
      121 aaatcatcaa agccgaatat aattaaaatg tattactagc taaagaaatc atcactaata
      181 tagaatgtag aatgaaccca tgtatattag atactaattg tatcgtaaga ctttcaaaag
      241 tctacaagac attaaatgac aagttgactt taaatttcaa ataaataatt tattttttct
      301 ataagcaata acatttttgc taaattaaga cttggtaatt aggtaatact attgttgttc
      361 tatggaatat tcgatcgaaa cattcttatc agtctcaaaa acttaaaaca aacttataat
      421 ataacccata tgttataacc cattgatgaa caaaaattag actctttggc cttagtcgac
      481 ggatccccga caccagacca actggtaatg gtagcgaccg gcgctcagct ggaattaggc
      541 cttctagacc gcggttaggg cccctagtat ttggtagcat tgcctttaaa ttgtttaact
      601 tgggtcaaac gtttcgggta gccttccaca agcttcccac aataatttgg gtgaattttg
      661 gcccattcct cctgacagag ctggtgtaac tgagtcaggt ttgtaggcct ccttgctcgc
      721 acacgctttt tcagttctgc ccacaaattt tctataggat tgaggtcagg gctttgtgat
      781 ggccactcca ataccttgac tttgttgtcc ttaagccatt ttgccacaac tttggaagta
      841 tgcttggggt cattgtcgtg ttggaagacc catttgcgac caagctttaa cttcctgact
      901 gatgtcttga gatgttgctt caatatatcc acatactgca ccgcgtccat gatgccatct
      961 attttgtgaa gtgcaccagt ccctcctgca gcaaagcacc cccacaacat gatgctgcca
     1021 cccccgtgct tcacggttgg gatggtgttc ttcggcttgc aagcctcccc cttcttcctc
     1081 caaacataac gatggtcatt atggccaaac agttctattt ttgtttcatc agaccagagg
     1141 acatttctcc aaaaagtacg atctttgtcc ccatgtgcag ttgcaaaccg tagtctggct
     1201 ttcttatgtc ggttttggag cagtggcttc ttccttgctg agtggccttt caggttatgt
     1261 cgatatagga ctcgttttac tgtggatata gatacttttg tacctgtttc ctccagcatc
     1321 ttcacaaggt cctttgctgt tgttctggga ttgatttgca cttttcgcac caaagtacgt
     1381 tcatctctag gagacagaac gcgtctcctt cctgagcggt atgacggctg cgtggtccca
     1441 tggtgtttat acttgcgtac tattgtttgt acagatgaac gtggtaccgc caggcgtttg
     1501 gaaattgctc ccaaggatga accagacttg tggaggtcta caattctttt tctgaggtct
     1561 tggctgattt cttttgattt tcccatggta ctagtcccta tagtgagtcg tattaattcc
     1621 gcggccgcag atctgttaac gaattcccaa ttccctattc agagttctct tcttgtattc
     1681 aataattact tcttggcaga tttcagtagt tgcagttgat ttacttggtt gctggttact
     1741 tttaattgat tcactttaac ttgcacttta ctgcagattg tttagcttgt tcagctgcgc
     1801 ttgtttattt gcttagcttt cgcttagcga cgtgttcact ttgcttgttt gaattgaatt
     1861 gtcgctccgt agacgaagcg ctctatttat actccggcgc tcttttcgcg aacattcgag
     1921 gcgcgctctc tcgaaccaac gagagcagta tgccgtttac tgtgtgacag agtgagagag
     1981 cattagtgca gagagggaga cccaaaaaga aaagagagaa taacgaataa cggccagaga
     2041 aatttctcga gttttcttct gccaaacaaa tgacctacca caataaccag tttgttttgg
     2101 gattctaggg ggatcgggga tcaattctag tatgtatgta agttaataaa accctttttt
     2161 ggagaatgta gatttaaaaa aacatatttt ttttttattt tttactgcac tggatatcat
     2221 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgaa gtaccaggtt
     2281 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
     2341 tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca
     2401 ccgtttgtag cgttacctag cgtcaatgtc cgccttcagt tgcactttgt cagcggtttc
     2461 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
     2521 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
     2581 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
     2641 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
     2701 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
     2761 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat cgcacaaaca aatagtgaca
     2821 cgaaacagat tattctggta gctgtgctcg ctatataaga caatttttaa gatcatatca
     2881 tgatcaagac atctaaaggc attcattttc gactacattc ttttttacaa aaaatataac
     2941 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
     3001 tcgtaggata cttcgttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     3061 agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg
     3121 cttcgggcag gccaaaaact acggcacgct cctgccaccc agtccgccgg aggactccgg
     3181 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
     3241 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
     3301 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
     3361 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
     3421 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
     3481 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
     3541 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
     3601 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
     3661 ctgattttcc aggccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
     3721 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
     3781 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
     3841 gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactca
     3901 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
     3961 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
     4021 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
     4081 tagtgagttc gatgtgttta ttaagggtat ctagcattac attacatctc aactcctatc
     4141 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     4201 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     4261 ttttgctatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatttgga
     4321 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     4381 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     4441 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     4501 ttcttggaaa caaattcgct agattttagt tagaattgcc tgattccaca cccttcttag
     4561 tttttttcaa tgagatgtat agtttatagt tttgcagaaa ataaataaat ttcatttaac
     4621 tcgcgaacat gttgaagata tgaatattaa tgagatgcga gtaacatttt aatttgcaga
     4681 tggttgccat cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggcgtga
     4741 tgaatatcaa cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg
     4801 ccacgataaa tgtaagtctt gtttagaata catttgcata ttaataattt actaactttc
     4861 taatgaatcg attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc
     4921 cgaagtcgac tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattaccg
     4981 ctttttctca cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg
     5041 gccggagtgc tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca
     5101 acgtccttcg gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg
     5161 ggtccgccgg ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg
     5221 gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt
     5281 ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac
     5341 accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat
     5401 ctgccgctgg actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat
     5461 ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc
     5521 ttgttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc
     5581 taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc
     5641 cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca
     5701 cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt
     5761 tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt
     5821 tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg
     5881 acgaccttgg agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttccgag
     5941 tggttcattt cgtctcaata gaaattagta ataaatattt gtatgtacaa tttatttgct
     6001 ccaatatatt tgtatatatt tccctcacag ctatatttat tctaatttaa tattatgact
     6061 ttttaaggta attttttgtg acctgttcgg agtgattagc gttacaattt gaactgaaag
     6121 tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt
     6181 gttgtttata tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa
     6241 tgcttggatt tcactggaac tagaattaat tcggctgctg ctctaaacga cgcatttcgt
     6301 actccaaagt acgaattttt tccctcaagc tcttattttc attaaacaat gaacaggacc
     6361 taacgcacag tcacgttatt gtttacataa atgatttttt ttactattca aacttactct
     6421 gtttgtgtac tcccactggt atagccttct tttatctttt ctggttcagg ctctatcact
     6481 ttactaggta cggcatctgc gttgagtcgc ctccttttaa atgtctgacc ttttgcaggt
     6541 gcagccttcc actgcgaatc tttaaagtgg gtatcacaaa tttgggagtt ttcaccaagg
     6601 ctgcacccaa ggctctgctc ccacaatttt ctcttaatag cacacttcgg cacgtgaatt
     6661 aattttactc cagtcacagc tttgcagcaa aatttgcaat atttcatttt tttttattcc
     6721 acgtaagggt taatgttttc aaaaaaaaat tcgtccgcac acaacctttc ctctcaacaa
     6781 gcaaacgtgc actgaattta agtgtatact tcggtaagct tcggctatcg acgggaccac
     6841 cttatgttat ttcatcatgg gccagaccca cgtagtccag cggcagatcg gcggcggaga
     6901 agttaagcgt ctccaggatg accttgcccg aactggggca cgtggtgttc gacgatgtgc
     6961 agctaatttc gcccggctcc acgtccgccc attggttaat cagcagaccc tcgttggcgt
     7021 aacggaacca tgagaggtac gacaaccatt tgaggtatac tggcaccgag cccgagttca
     7081 agaagaaggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7261 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     7321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7501 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7921 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7981 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8161 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8221 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8281 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8341 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8401 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8461 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8521 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8581 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8641 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8701 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8821 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     8881 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     8941 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9001 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9061 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcacc
     9121 gaatcgcgcg gaactaacga cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg
     9181 cggagagcgg gtgggagaca gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa
     9241 ttatgaagag ggcgcgcgat ttgaaaagta tgtatataaa aaatatatcc cggtgtttta
     9301 tgtagcgata aacgagtttt tgatgtaagg tatgcaggtg tgtaagtctt ttggttagaa
     9361 gacaaatcca aagtctactt gtggggatgt tcgaagggga aatacttgta ttctataggt
     9421 catatcttgt ttttattggc acaaatataa ttacattagc tttttgaggg ggcaataaac
     9481 agtaaacacg atggtaataa tggtaaaaaa aaaaacaagc agttatttcg gatatatgtc
     9541 ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac ccggaagctc
     9601 acgatgagaa tggccagacc atgatgaaat aacataaggt ggtcccgtcg gcaagagaca
     9661 tccacttaac gtatgcttgc aataagtgcg agtgaaagga atagtattct gagtgtcgta
     9721 ttgagtctga gtgagacagc gatatgattg ttgattaacc cttagcatgt ccgtggggtt
     9781 tgaattaact cataatatta attagacgaa attattttta aagttttatt tttaataatt
     9841 tgcgagtacg ca
//
