LOCUS       sequence-117821-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for mFTH1 knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBGO
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..43
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(682..701)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 ctcgagtgcc accatgtacc catacgatgt tccagattac gctatgacca ccgcgtctcc
       61 ctcgcaagtg cgccagaact accaccagga cgcggaggct gccatcaacc gccagatcaa
      121 cctggagttg tatgcctcct acgtctatct gtctatgtct tgttattttg accgagatga
      181 tgtggctctg aagaactttg ccaaatactt tctccaccaa tctcatgagg agagggagca
      241 tgccgagaaa ctgatgaagc tgcagaacca gcgaggtggc cgaatcttcc tgcaggatat
      301 aaagaaacca gaccgtgatg actgggagag cgggctgaat gcaatggagt gtgcactgca
      361 cttggaaaag agtgtgaatc agtcactact ggaactgcac aaactggcta ctgacaagaa
      421 tgatccccac ttatgtgact tcattgagac gtattatctg agtgaacagg tgaaatccat
      481 taaagaactg ggtgaccacg tgaccaactt acgcaagatg ggtgcccctg aagctggcat
      541 ggcagaatat ctctttgaca agcacaccct gggacacggt gatgagagct aactcgagga
      601 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
      661 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
      721 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
      781 ttg
//
