LOCUS       sequence-117827-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inntag 5: a small self-structured epitope aimed at innocuous protein
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Georgieva MV, Yahya G, Codo L, Ortiz R, Teixido L, Claros J, Jara R,
            Jara M, Iborra A, Gelpi JL, Gallego C, Orozco M, Aldea M
  TITLE     Inntags: small self-structured epitopes for innocuous protein 
            tagging.
  JOURNAL   Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556.
  PUBMED    26322837
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556."
COMMENT     Sequence Label: pBS-IT5
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..57
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     41..57
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(403..422)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(404..422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(432..449)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(432..448)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(441..463)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(677..696)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagaacta gtggatcccc
       61 cgggctgcag gaattctggt gccgaaagtg acctttaccg tggaaaaagg cagcaacgaa
      121 aaacatctgg ccgtgctggt gaaatatgaa ggcgatacca tggccgaagt ggaactgcgc
      181 gaacatggca gcgatgaatg ggtggccatg accaaaggcg aaggcggcgt gtggaccttt
      241 gatagcgaag aaccgctgca gggcccgttt aactttcgct ttctgaccga aaaaggcatg
      301 aaaaacgtgt ttgatgatgt ggtgccggaa aaatatacca ttggcgccac ctatgccccg
      361 gaagaacagt cgacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat
      421 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      481 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      541 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
      601 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      661 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      721 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      781 cacctcgacc ccaaaa
//
