LOCUS       sequence-117828-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inntag 6: a small self-structured epitope aimed at innocuous protein
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Georgieva MV, Yahya G, Codo L, Ortiz R, Teixido L, Claros J, Jara R,
            Jara M, Iborra A, Gelpi JL, Gallego C, Orozco M, Aldea M
  TITLE     Inntags: small self-structured epitopes for innocuous protein 
            tagging.
  JOURNAL   Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556.
  PUBMED    26322837
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556."
COMMENT     Sequence Label: pBS-IT6
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..64
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     48..64
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(251..270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(252..270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(280..297)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(280..296)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(289..311)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      438..818
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(525..544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     735..756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgctct agaactagtg
       61 gatcccccgg gctgcaggaa ttctggaaca gtgcggccgc caggccggcg gcaaactgtg
      121 cccggataac ctgtgctgca gccagtgggg ctggtgcggc agcaccgatg aatattgcag
      181 cccggatcat aactgccaga gcaactgcaa agatcagtcg acctcgaggg ggggcccggt
      241 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
      301 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      361 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      421 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      481 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      541 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      601 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      661 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      721 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
      781 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa a
//
