LOCUS       sequence-117829-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inntag 10: a small self-structured epitope aimed at innocuous 
            protein tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Georgieva MV, Yahya G, Codo L, Ortiz R, Teixido L, Claros J, Jara R,
            Jara M, Iborra A, Gelpi JL, Gallego C, Orozco M, Aldea M
  TITLE     Inntags: small self-structured epitopes for innocuous protein 
            tagging.
  JOURNAL   Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556.
  PUBMED    26322837
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556."
COMMENT     Sequence Label: pBS-IT10
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..60
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     44..60
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(238..257)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(239..257)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(267..284)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(267..283)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(276..298)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      425..805
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(512..531)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     722..743
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctaaagggaa caaaagctgg agctccaccg cggtggcggc cgctctagaa ctagtggatc
       61 ccccgggctg caggaattct ggatgatggc ctgtgctatg aaggcaccaa ctgcggcaaa
      121 gtgggcaaat attgctgcag cccgattggc aaatattgcg tgtgctatga tagcaaagcc
      181 atttgcaaca aaaactgcac ccagtcgacc tcgagggggg gcccggtacc caattcgccc
      241 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
      301 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      361 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      421 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      481 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
      661 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
      721 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      781 ttataaggga ttttgccgat ttcggcctat tggttaaa
//
