LOCUS       sequence-117845-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Functions as a genetic Cre reporter that switches from DsRed 
            expression to eGFP expression upon presence of Cre recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Zomer A, Maynard C, Verweij FJ, Kamermans A, Schafer R, Beerling E, 
            Schiffelers RM, de Wit E, Berenguer J, Ellenbroek SI, Wurdinger T, 
            Pegtel DM, van Rheenen J
  TITLE     In Vivo imaging reveals extracellular vesicle-mediated phenocopying 
            of metastatic behavior.
  JOURNAL   Cell. 2015 May 21;161(5):1046-57. doi: 10.1016/j.cell.2015.04.042.
  PUBMED    26000481
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.04"; journalName: "Cell"; date: "2015-05-21- 
            21"; volume: "161"; issue: "5"; pages: "1046-57"
COMMENT     Sequence Label: pLV-CMV-LoxP-DsRed-LoxP-eGFP
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     221..242
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      284..293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    344..377
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(464..487)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(662..681)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
       61 gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc
      121 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
      181 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
      241 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgtagtcg
      301 aattcgttaa cctcgagaga tctaacgggc cctctagact cgaataactt cgtataatgt
      361 atgctatacg aagttattcg agcggcctta ttcagctagc tctactggaa caggtggtgg
      421 cgggcctcgg cgcgctcgta ctgctccacc acggtgtagt cctcgttgtg ggaggtgatg
      481 tccagcttgg agtccacgta gtagtagccg ggcagcttca cgggcttctt ggccatgtag
      541 attgacttga actccaccag gtagtggccg ccgcccttca gcttcagcgc cttgtggatc
      601 tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg tggagggctc ccagcccaga
      661 gtcttcttct gcattacggg gccgtcggag gggaagttca cgccgatgaa cttc
//
