LOCUS       sequence-117853-         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expresses CRE excisable human MyoD and dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Gersbach C
  TITLE     Multiplex CRISPR/Cas9 Genome Engineering for Directing Myogenic 
            Cellular Differentiation in Primary Human Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV floxed hMyoD-IRES-dsRed
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    154..616
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(314..333)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     543..560
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tgcagcttgg cggtctgggt gccctcgtag ggcttgccct cgccctcgcc ctcgatctcg
       61 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa gggcttgatg
      121 acgttctcag tgctatccat ggttgtggcc atattatcat cgtgtttttc aaaggaaaac
      181 cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa
      241 gcatgtgcac cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt
      301 cagccccttg ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact
      361 cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg
      421 gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga
      481 cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc
      541 ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc
      601 aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac
      661 aaacgcacac cggccttatt ccaagcggct tc
//
