LOCUS       sequence-117868-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for yeast expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pDRFLIP34
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..58
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     43..59
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(97..126)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga
       61 taagcttgat atcgaattcc tgcagcccgg cccttataaa tcttcctcac ttatcaattt
      121 ttgttcggag ttgcgggcca cggcgctctc gtaaacggtc accttgttgt agtccttgtc
      181 gtggttcagg atctcgatgc ggtggtccac aaagtgatag tcgggcagct tcaccgcctt
      241 cttggccctg tagatggtct tgaagtcaac gcggtggtgg ccgccgccct ccagcagcag
      301 cttgtgcttg acgtcgccct tcagcacgcc gtcgcgcacg tacatcctct cggtggaggc
      361 gtcccagccg gtggtcttct tctgcatcac ggggccgttg ggggggaagt tctcgccctt
      421 gaggtgtatc tcgtagatga aggagtcctc ctccatggag atgtcggact tcaccttcac
      481 gatgcccttg tcctcgaagg tcatggtgcg ctcccaagag tagccctcgg ggaaggactg
      541 cttgaagtag ttggggatgt cgtcggggta cttggtgaag gccctgttgc cgtaggcgaa
      601 cgcggtggtc agaatgtcgt aggagaaggg caggggggct ccctccttca cctccaggtt
      661 gatggtgttg gtgccgtcgt agggcttgcc ctcgccctcg ccctcgatca cgaa
//
