LOCUS       sequence-117880-        1623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression construct of human codon-adapted anion 
            channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via 
            a NotI site in the pFUGW vector backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL
  TITLE     Natural light-gated anion channels: A family of microbial rhodopsins
            for advanced optogenetics.
  JOURNAL   Science. 2015 Jun 25. pii: aaa7484.
  PUBMED    26113638
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Jun 25. pii: aaa7484."
COMMENT     Sequence Label: pFUGW-hGtACR1-EYFP
FEATURES             Location/Qualifiers
     source          1..1623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(901..903,904..1617)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(943..964)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1204..1223)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1551..1572
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatccatga gcagcatcac ctgtgatccc gccatctacg gcgaatggag cagggagaac
       61 cagttctgcg tggagaagag cctgatcacc ctggacggca tcaagtacgt ccagctggtg
      121 atggccgtcg tgagcgcctg tcaggtgttc ttcatggtga ccagagcccc caaggtgccc
      181 tgggaagcca tctacctgcc caccaccgag atgatcacct attccctggc cttcaccgga
      241 aacggctaca tcagagtggc caatggcaag tacctgccct gggccagaat ggccagctgg
      301 ctgtgcacct gccctatcat gctgggcctg gtgagcaata tggccctcgt gaagtacaag
      361 agcatccccc tcaaccctat gatgatcgcc gcctccagca tctgcaccgt gttcggcatc
      421 accgcctccg tggtgctaga cccgctgcac gtgtggctgt actgtttcat cagcagcatc
      481 ttcttcatct tcgagatggt ggtggccttc gccattttcg ccattaccat ccacgatttc
      541 cagaccatcg gctcccccat gtccctgaag gtggtggaga ggctgaagct gatgaggatc
      601 gtgttctacg tgagctggat ggcctaccct atcctgtgga gcttctccag caccggcgcc
      661 tgcatcatga gcgagaacac cagcagcgtg ctgtacctgc tgggcgacgc tctgtgcaag
      721 aacacctacg gcatcctgct gtgggctaca acctggggcc tgctgaacgg caagtgggac
      781 agggattacg tgaagggcag gaacgtggac ggcaccctga tgcctgagta cgagcaggac
      841 ctggagaagg gcaacaccga gaggtacgag gacgccagag ccggcgagac cgcggccgcc
      901 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      961 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1021 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1081 gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     1141 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1201 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1261 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1321 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1381 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1441 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1501 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1561 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagaa
     1621 ttc
//
