LOCUS       sequence-117882-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses intein-Cas9(S219-G521R) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Davis KM, Pattanayak V, Thompson DB, Zuris JA, Liu DR
  TITLE     Small molecule-triggered Cas9 protein with improved genome-editing 
            specificity.
  JOURNAL   Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. 
            Epub 2015 Apr 6.
  PUBMED    25848930
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1793"; journalName: "Nat Chem Biol"; date: 
            "2015-05"; volume: "11"; issue: "5"; pages: "316-8"
COMMENT     Sequence Label: pKMD111e - intein-Cas9(S219-G521R)
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcacggaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
       61 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaaga
      121 atggagcatc tgtacagcat gaagtacaag aacgtggtgc ccctctatga cctgctgctg
      181 gagatgctgg acgcccaccg cctacatgcg ggtggttctg gtgctagccg cgtgcaggcg
      241 ttcgcggatg ccctggatga caaattcctg cacgacatgc tggcggaagg actccgctat
      301 tccgtgatcc gagaagtgct gccaacgcgg cgggcacgaa cgttcgacct cgaggtcgag
      361 gaactgcaca ccctcgtcgc cgaaggggtt gtcgtgcaca actgccgacg gctagaaaac
      421 ctgatcgcac aattacccgg agagaagaaa aatgggttgt tcggtaacct tatagcgctc
      481 tcactaggcc tgacaccaaa ttttaagtcg aacttcgact tagctgaaga tgccaaattg
      541 cagcttagta aggacacgta cgatgacgat ctcgacaatc tactggcaca aattggagat
      601 cagtatgcgg acttattttt ggctgccaaa aaccttagcg atgcaatcct cctatctgac
      661 atactgagag ttaatactga gattaccaag gcgccgttat ccgcttcaat gatcaaaagg
      721 tacgatgaac atcaccaaga cttgacactt ctcaaggccc tagtccgtca gcaactgcct
      781 ga
//
