LOCUS       sequence-117883-        1611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression construct of human codon-adapted anion 
            channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via 
            a NotI site in the pFUGW vector backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL
  TITLE     Natural light-gated anion channels: A family of microbial rhodopsins
            for advanced optogenetics.
  JOURNAL   Science. 2015 Jun 25. pii: aaa7484.
  PUBMED    26113638
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Jun 25. pii: aaa7484."
COMMENT     Sequence Label: pFUGW-hGtACR2-EYFP
FEATURES             Location/Qualifiers
     source          1..1611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(889..891,892..1605)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(931..952)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1192..1211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1539..1560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatccatgg cctcccaggt cgtgtacggc gagtgggcca gcacccacac agagtgctac
       61 aacatgagca ggatcgacag caccttcgtg agcctgctgc agctggtgtg ggccgtggtg
      121 agcggatgcc agaccatctt catgatcagc agggccccca aggtgccctg ggagagcgtg
      181 tacctgccct tcgtggagtc catcacatac gccctggcca gcaccggcaa tggcacactg
      241 cagatgaggg acggcaggtt ctttccctgg tccaggatgg ccagctggct gtgcacatgc
      301 cccattatgc tgggccagat ctccaacatg gctctggtga agtacaagag catccctctg
      361 aaccccatcg cccaggccgc cagcattatc agggtggtga tgggcattac cgctaccatc
      421 tcccccgccg agtacatgaa gtggctgttc ttcttcttcg gcgctacctg cctggtcttc
      481 gaatacagcg tggtgttcac catcttccag gtgggactgt acggcttcga gtccgtgggc
      541 acccctctgg cccagaaggt ggtggtgagg atcaagatgc tgaggctgat cttcttcatc
      601 gcctggacca tgttccccat cgtgtggctg atttccccca ccggcgtgtg cgtgatccac
      661 gagaacgtga gcgccatcct gtatctgctc gccgacggcc tgtgcaagaa cacctacggc
      721 gtgatcctgt ggtccaccgc ctggggagtg ctcgagggaa agtgggaccc ggcttgcctc
      781 cctggccagg agaagcctga ggccgacgac cccttcggac tgaaccacga gaagaatgcc
      841 ccccccaacg acgaggtcaa catcagaatg ttcggcaggg cggccgccgt gagcaagggc
      901 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      961 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1021 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     1081 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1141 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1201 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1261 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1321 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1381 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1441 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1501 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1561 accgccgccg ggatcactct cggcatggac gagctgtaca agtaagaatt c
//
