LOCUS       sequence-117886-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PFKL
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      792..801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttgggcag aagcccttgt ccatgctcag
      121 ggtgcggcgg gtcacgtgct ccagctcccc tgacacgtag gcggccatac tgatgcggta
      181 ttgtgccagc atcttcagca tgagccgcag gctcagccac cactgctccc gtggcatgcg
      241 gtgctcgaaa tcagtgtctt tcttgagctc agtgacgggg ctgaaggcca ccgccttctt
      301 cttcaggccg atcacgcagg ccgagtctgg ggcattggcg aacacccgtc ccttgcggta
      361 aacctcgcgc agcttctccg acaaccacag catggccttc acccccagct tggtcccata
      421 gttccggtca aagggggttg gagcgccacc ctgctgcagg tggcccagga cattggtcct
      481 gcagtcgaag acgcccttgc cctctgatga gtacaggttg tacaggaact ccgtggtgta
      541 gtagtcatgg cacttctcgt tccgcagcac caggcccctc tgaatgtctg tcttcatctt
      601 ctccgtcatg tgctccacgt tgacctttaa gtcgtggatg ttgaaagggt cctcgaagac
      661 gtaggcggcg tcggccccca cagcaatgcc agtcacggtg gccaggtagc cacagtaacc
      721 ccccatggtc tccacgatga acacacggcg cttggtcccc gaggcagact gtttgatgcg
      781 gtcacagctc tccatggcgg catttacagc agtgtcggag cccaggctga agtcggtgcc
//
