LOCUS       sequence-117887-         578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV/TO 
            promoter, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV/TO Neo DEST (685-3)
FEATURES             Location/Qualifiers
     source          1..578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(87..93,94..111,112..117))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(142..266)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    307..325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    328..346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(348..551)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(377..397)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
       61 cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc
      121 tggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta
      181 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
      241 cgttcagctt ttttgtacaa acttgttgat atctgcagaa ttccaccaca ctggactagt
      301 cgacgatctc tatcactgat agggagatct ctatcactga tagggagagc tctgcttata
      361 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      421 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      481 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      541 aaccgcatca ccatggtaat agcgatgact aatacgta
//
