LOCUS       sequence-117890-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Fluorescence Lifetime Imaging Microscopy 
            (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLIM-AKAR
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcttac tcgatgttgt
       61 gcctgatttt gaagttgacc ttgataccgt ttttctgctt gtcggccata atatagacgt
      121 tcacgctatt ccagttgtac tccagcttat gcccgaggat atttccatct tctttaaagt
      181 caatcccctt cagctcgatt cggttgacga gggtgtcacc ttcgaacttc acctctgcgc
      241 gagttttgta gttgccatcg tccttaaaga aaattgtccg ttcctgcacg tatccctcag
      301 gcatggcaga tttaaagaaa tcatgctgct tcatgtggtc ggggtacctt gcaaagcaca
      361 tcaggccgta accgaaggta gtgaccaggg ttggccaagg cacgggcagc ttccctgtgg
      421 tgcagatcag tttgagtgtc agctttccat aggtagcatc cccttctccc tcgccgctca
      481 cgctgaactt gtggccgttc acgtcgccgt ccagttccac gagaatgggg accacgccag
      541 tgaacagttc ctcgcccttg gacaccatgc taccccctgt tccgcctttg tacagctcgt
      601 ccatcccgag agtgattcca gcggctgtga ctctttcgag cagcaccata tgatcccttt
      661 tctcatttgg atcttttgac agggcactct gataagagag gtagtggttg tctgggagga
      721 ggactggccc gtcacc
//
