LOCUS       sequence-117902-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vimentin-targeted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Vimentin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..675
                     /codon_start=1
                     /product="fast-switching photoswitchable fluorescent 
                     protein useful for fluorescence nanoscopy (Tiwari et al., 
                     2015)"
                     /label=Kohinoor
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSL
                     EGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSGLPRQ
                     AK"
ORIGIN
        1 atgagtgtga ttaaaccaga catgaagatc aagctgcgta tggaaggcgc tgtaaatgga
       61 cacccgttcg cgattgaagg agttggcctt gggaagcctt tcgagggaaa acagagtatg
      121 gaccttaaag tcaaagaagg cggacctctg cctttcgcct atgacatctt gacaatggcc
      181 ttctgttacg gcaacagggt attcgccaaa tacccagaaa atatagtaga ttatttcaag
      241 cagtcgtttc ctgagggcta ctcttgggaa cgaagcatga tttacgaaga cgggggcatt
      301 tgtatcgcga caaacgacat aaccctggat ggtgactgtt atatctatga aattcgattt
      361 gatggtgtga actttcctgc caatggtcca gttatgcaga agaggactgt gaaatgggag
      421 ccatccactg agaaattgta tgtgcgtgat ggagtgctga agagcgatgg caattacgct
      481 ctgtcgcttg aaggaggtgg ccactaccga tgtgactcca aaactactta taaagctaag
      541 aaggttgtcc agttgccaga ctatcacgat gtggtccacc acattgagat taaaagccac
      601 gacagagatt acagtaatgt taatctgcat gagcatgccg aagcgcattc tgggctgccg
      661 aggcaggcca agtaa
//
