LOCUS       sequence-117909-         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72214-BBa_J72090
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..27
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cggttctcca ttttttttac ctccttaaag atcatcaatt cacgcgacca atcttgtacc
       61 aaacattctg tttacgctgg atttgttgca gacgatcgta cggctcgctc gtaaagtaca
      121 gtttctcggc catatccggc ggatacaggc ccggaaactt ggaatgcatg taacgcgtgt
      181 aacgcgcacg catgaaaaag atcgggttcg agcagctcaa aacgtcatag ttggcgcgag
      241 ccatgtcttg cattgcgtct gcctggactt tgcgcagctc cgtgaactcc ggaaaggctt
      301 tgtcttggtc gccctggtgg cggtccagca gctcgacaaa cgtgcgggcg tcctccagcg
      361 ccatgttcat accctgaccc aggaacggcg cagtcgcatg cgcagcatca cccagcaaca
      421 gcacattacc cttatagtga aaggtgctag agcgcacgtt aatcaggtcg ttgctcggct
      481 tcgccagaaa ctgacgcagc atttcgtcac gcgcgtcacg cggcaggcca ccgaagtaac
      541 gatcgaagaa cgcacgcatc gtcggttcgt cggtggtcgt cagggaaggg ctacccgagt
      601 acggcaggca aacggcgatg ctgacgctac catctgggat cgtagccgca cga
//
