LOCUS       sequence-117910-       12793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72214-BBa_J72090
FEATURES             Location/Qualifiers
     source          1..12793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             17..25
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             1291..1299
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             4311..4319
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             5624..5632
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      complement(6231..6325)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6346..7005)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6920..6939
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(7266..7283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7400..7945)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7480..7499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    8025..8049
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(11185..11212)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(11228..11299)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(11551..11835)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(11609..11628)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             11862..12740
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 gaattcatga gatctttaag gaggtaaaaa aaatgaaaca ttcttccgat atctgcattg
       61 ttggtgctgg tatttctggt ttgacgtgcg caagccatct gctggacagc ccggcatgcc
      121 gtggtctgag cctgcgtatc tttgacatgc agcaagaagc cggtggccgt atccgcagca
      181 aaatgctgga tggtaaggca agcattgaac tgggcgcagg tcgctactcc cctcagttgc
      241 acccgcattt ccaaagcgca atgcagcact atagccaaaa gagcgaagtc tatccgttca
      301 cccagttgaa gttcaaatct cacgtgcagc aaaagctgaa gcgcgccatg aatgaactgt
      361 ccccgcgtct gaaagagcat ggtaaagaga gctttttgca gtttgtcagc cgttatcaag
      421 gtcacgatag cgcggttggt atgatccgct ctatgggtta cgacgcactg ttcctgccgg
      481 atatcagcgc agaaatggcc tacgacattg tgggtaagca cccggagatc cagagcgtga
      541 cggacaacga cgcgaaccaa tggtttgcag cggaaacggg ctttgctggt ctgattcagg
      601 gcatcaaggc taaggttaag gcggcaggtg cgcgttttag cctgggttat cgtctgctga
      661 gcgtccgtac cgacggtgac ggctacctgc tgcaactggc aggtgacgac ggctggaaac
      721 tggagcaccg tacccgccat ctgattctgg cgattccgcc gagcgcgatg gcgggtttga
      781 atgttgattt tccagaagcc tggtccggtg cgcgctatgg cagcctgccg ctgtttaagg
      841 gctttctgac gtacggtgag ccgtggtggt tggactacaa actggacgat caggtgctga
      901 ttgttgacaa cccgctgcgc aaaatctatt tcaaaggcga taagtacctg ttcttctata
      961 ccgatagcga gatggcgaat tactggcgcg gttgtgtcgc ggagggcgag gacggttacc
     1021 tggagcaaat tcgcacccat ttggctagcg cactgggtat cgtccgtgaa cgtatcccgc
     1081 aaccgctggc acacgttcac aagtattggg cgcacggcgt tgagttttgc cgtgattctg
     1141 atattgacca cccgagcgca ctgtctcatc gcgacagcgg tatcatcgcg tgctccgatg
     1201 cgtacacgga gcattgtggt tggatggagg gcggtctgct gagcgcccgt gaggcaagcc
     1261 gtctgctgtt gcagcgtatc gccgcgtgat taaggaggta aaaaaaatga gcattctgga
     1321 tttcccgcgt atccacttcc gtggctgggc ccgtgtcaat gcgccgaccg cgaaccgcga
     1381 tccgcacggc cacatcgata tggccagcaa taccgtggcg atggcgggtg agccgttcga
     1441 cctggcacgc catcctacgg agttccaccg tcacctgcgc tccctgggtc cgcgcttcgg
     1501 cttggatggt cgtgctgacc cggaaggccc gttcagcctg gccgagggct acaacgctgc
     1561 cggtaacaac cacttttcgt gggagagcgc aaccgttagc cacgtgcaat gggatggcgg
     1621 tgaggcggat cgtggtgacg gtctggtcgg tgctcgtttg gcactgtggg gtcactacaa
     1681 tgattatctg cgtaccacct tcaatcgtgc tcgttgggtc gacagcgacc cgacgcgccg
     1741 tgacgctgca caaatctatg cgggccaatt caccattagc ccggctggtg ccggtccggg
     1801 tacgccgtgg ctgtttacgg cagacattga tgatagccat ggtgcacgtt ggacgcgtgg
     1861 cggccacatt gcagagcgtg gcggccactt cttggatgaa gagtttggtc tggcacgcct
     1921 gtttcagttc tctgtgccga aagatcaccc acattttctg tttcacccgg gtccgtttga
     1981 ttccgaggcc tggcgtcgtc tgcaattggc tctggaggat gacgacgttc tgggtctgac
     2041 cgtgcaatat gcgttgttca atatgagcac cccgcctcag ccgaacagcc cggtttttca
     2101 cgatatggtc ggtgttgtcg gtctgtggcg tcgtggtgaa ctggcgagct acccggctgg
     2161 tcgtctgctg cgtccgcgtc aaccgggtct gggtgacctg accctgcgcg tcaacggtgg
     2221 tcgcgttgcg ctgaatttgg cgtgtgccat tccgttcagc actcgtgccg cgcagccaag
     2281 cgcaccggac cgcctgaccc cggacctggg tgccaaactg ccgctgggcg atctgctgct
     2341 gcgtgatgag gacggcgcac tgttggcacg tgtgccgcag gctctgtacc aagactattg
     2401 gacgaatcac ggtattgtgg acctgccgct gctgcgcgaa ccgcgtggta gcttgaccct
     2461 gagcagcgaa ctggcggagt ggcgtgagca agactgggtc acccaaagcg acgcgtctaa
     2521 cctgtacctg gaggcaccgg atcgccgtca cggtcgcttt ttccctgaga gcatcgcgct
     2581 gcgcagctac tttcgcggtg aagcgcgtgc gcgtccggat atcccgcatc gtatcgaggg
     2641 catgggcctg gtcggcgtcg aatctcgtca ggatggcgac gctgcggaat ggcgtctgac
     2701 gggtctgcgt ccgggtccgg cacgcattgt tctggacgat ggtgccgagg cgatccctct
     2761 gcgtgttctg cctgacgatt gggcgctgga tgacgcgacc gtcgaagaag tggattacgc
     2821 ctttttgtac cgccacgtta tggcgtatta cgagctggtg tatccattca tgagcgacaa
     2881 ggtgttttcc ctggctgatc gttgcaaatg tgaaacgtac gcacgtctga tgtggcagat
     2941 gtgtgatccg cagaaccgca acaagtccta ttacatgccg agcacccgcg aactgtcggc
     3001 accgaaagct cgtttgttct tgaagtatct ggcccacgtg gaaggccagg cacgcctgca
     3061 agcacctccg ccagcgggtc cggcacgcat tgaatctaaa gcccagttgg cggcagagct
     3121 gcgtaaagcc gtcgacctgg agctgtctgt gatgctgcaa tacctgtacg cggcgtatag
     3181 cattccgaac tatgcacagg gccaacaacg tgttcgtgac ggtgcgtgga ccgccgagca
     3241 gctgcaactg gcgtgcggta gcggtgaccg tcgccgtgat ggcggtattc gtgcagcact
     3301 gctggaaatt gctcatgaag aaatgattca ttacctggtc gttaacaacc tgctgatggc
     3361 cctgggcgag ccgttctacg cgggtgtccc gctgatgggc gaagcggcac gtcaggcgtt
     3421 tggcctggac accgagttcg ctctggaacc gtttagcgaa agcacgctgg cacgttttgt
     3481 tcgtctggaa tggccgcact ttatcccagc accgggcaaa tccatcgcgg actgctatgc
     3541 cgccattcgt caggcgtttt tggatctgcc ggacttgttt ggtggcgagg caggtaagcg
     3601 tggcggtgaa caccacctgt tcctgaatga gctgaccaac cgtgcgcatc cgggttatca
     3661 actggaagtt ttcgatcgcg actcggcgct gtttggtatt gcatttgtga ccgatcaggg
     3721 cgaaggtggc gctctggaca gcccgcacta cgaacatagc cattttcaac gtctgcgtga
     3781 aatgagcgcg cgtatcatgg ctcaaagcgc accgttcgaa ccggcgctgc cggcgttgcg
     3841 taatccggtt ctggatgaga gcccgggttg ccaacgtgtc gcagacggtc gtgcgcgtgc
     3901 gctgatggca ttgtaccaag gcgtttatga gctgatgttt gcgatgatgg cgcagcactt
     3961 cgccgtgaaa ccgctgggta gcttgcgtcg cagccgcctg atgaacgcag caatcgatct
     4021 gatgaccggt ctgttgcgtc cgctgagctg cgcgctgatg aacctgccaa gcggcatcgc
     4081 cggtcgcacg gccggtccgc cgctgccggg tccggttgac acccgtagct atgacgacta
     4141 cgcgctgggc tgtcgcatgc tggcacgccg ttgcgagcgt ctgctggagc aggcgagcat
     4201 gctggaaccg ggttggctgc cggatgcgca gatggagctg ctggatttct atcgtcgcca
     4261 aatgctggac ttggcgtgcg gcaaactgag ccgcgaggcc taatgatcct taaggaggta
     4321 aaaaaaatga aacgtgcgat tatcgttggt ggcggcctgg cgggtggcct gaccgcgatc
     4381 tacctggcga agcgtggcta cgaagtgcac gtcgtggaga agcgtggtga tcctctgcgc
     4441 gatctgagct cttacgtgga cgttgttagc agccgtgcga tcggcgtgag catgaccgtt
     4501 cgtggtatca agagcgtttt ggctgcgggc attccgcgtg cagagctgga tgcgtgtggc
     4561 gaaccgatcg tggcaatggc tttctccgtg ggtggtcagt atcgcatgcg cgaactgaag
     4621 ccgttggagg atttccgtcc gctgagcttg aaccgtgcgg cgtttcaaaa gctgctgaac
     4681 aaatacgcga acctggcagg cgttcgttac tactttgagc ataagtgcct ggatgttgac
     4741 ctggatggta agagcgtgtt gattcagggc aaagatggtc agccgcagcg tctgcaaggt
     4801 gacatgatta tcggtgcgga tggcgcccac agcgccgtcc gtcaggcgat gcagagcggc
     4861 ctgcgtcgtt tcgagttcca gcaaacgttc ttccgccatg gctacaaaac cctggttttg
     4921 ccggacgcgc aagcactggg ttaccgtaaa gacacgctgt actttttcgg catggattcc
     4981 ggtggcctgt tcgcgggtcg tgcggctacg atcccagatg gtagcgtcag catcgccgtt
     5041 tgcctgccgt actcgggtag cccttccctg acgaccaccg acgaaccgac gatgcgtgcg
     5101 ttcttcgatc gttacttcgg tggcctgccg cgtgacgcgc gtgacgaaat gctgcgtcag
     5161 tttctggcga agccgagcaa cgacctgatt aacgtgcgct ctagcacctt tcactataag
     5221 ggtaatgtgc tgttgctggg tgatgctgcg catgcgactg cgccgttcct gggtcagggt
     5281 atgaacatgg cgctggagga cgcccgcacg tttgtcgagc tgctggaccg ccaccagggc
     5341 gaccaagaca aagcctttcc ggagttcacg gagctgcgca aagtccaggc agacgcaatg
     5401 caagacatgg ctcgcgccaa ctatgacgtt ttgagctgct cgaacccgat ctttttcatg
     5461 cgtgcgcgtt acacgcgtta catgcattcc aagtttccgg gcctgtatcc gccggatatg
     5521 gccgagaaac tgtactttac gagcgagccg tacgatcgtc tgcaacaaat ccagcgtaaa
     5581 cagaatgttt ggtacaagat tggtcgcgtg aattgatgat ctttaaggag gtaaaaaaaa
     5641 tggagaaccg tgagccacca ctgttgccag cccgttggag cagcgcctat gtctcttatt
     5701 ggagcccgat gctgccggat gaccagctga ccagcggcta ttgctggttc gactatgaac
     5761 gtgacatctg tcgtattgac ggcctgttca atccgtggag cgagcgtgat actggttatc
     5821 gcctgtggat gtcggaggtt ggtaatgcgg ccagcggccg tacctggaaa caaaaagtcg
     5881 cctatggtcg tgagcgtacc gccctgggtg aacagctgtg tgagcgtccg ctggatgatg
     5941 agactggccc ttttgccgaa ttgttcctgc cacgcgatgt cctgcgccgt ctgggtgccc
     6001 gtcacattgg ccgtcgcgtg gttctgggtc gcgaagcgga cggttggcgt taccagcgcc
     6061 caggtaaagg tccgagcacc ctgtacctgg atgcggcgag cggcactcca ctgcgcatgg
     6121 tcaccggcga tgaagcgtcg cgtgcaagcc tgcgtgattt tccgaatgtg agcgaggcgg
     6181 agatcccgga cgcggttttc gcggccaagc gctaaggatc cgaggcttgg attctcacca
     6241 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     6301 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     6361 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     6421 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     6481 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     6541 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     6601 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     6661 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     6721 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     6781 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     6841 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     6901 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     6961 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     7021 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     7081 aaagttggaa cctcttacgt gcccgatcaa agatccgcac cgccggacat cagcgctagc
     7141 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
     7201 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
     7261 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
     7321 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
     7381 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
     7441 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7501 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     7561 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     7621 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
     7681 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
     7741 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
     7801 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
     7861 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
     7921 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta
     7981 aggcctcccc tgattctgtg gataaccggg atctgtaagg atcaaccact ttgtacaaga
     8041 aagctgggtc gaattgagat ccgaacggtt tattacgtac atcaggtaaa actgaccgat
     8101 aagccgcttt cttttgggta tagtgtcgtg gacagtcatt catctttctg cccctccaaa
     8161 agtaaaaacc cgccgaagcg ggtttttacg taaaacaggt gaaactgacc gataagccgc
     8221 tttcttttgg gtatagtgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa
     8281 acccgccgaa gcgggttttt acgtaaacca ggtgaaactg accgataagc cgctttcttt
     8341 tgggtatagc gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc
     8401 gaagcgggtt tttacgtaaa tcaggtgaaa ctgaccgata agccgggttc tgtcgtggac
     8461 agtcattcat ctaggccagc aatcgctcag atccttaatc atcatccccg tacagtttgc
     8521 gggtaacttc ttcgtcttcc tcctcgtctt cgtccagatc ctgttcaagc catgcttcgt
     8581 ttgatatgcc ttccgcatcg acatcaccat aaccacctgt atcgacgata tcggcaattt
     8641 cttctctacg atgctcaatc cacaatgcgg catcagcgcg gcggctggcg gcccgttctc
     8701 gcgcgatttt atccagatcc tcaagtgatg gagcgccaga tgctgtttgg ttttcctcat
     8761 catcggtatt ggtcttagga gcacgcagat cggctttgat ttgctccagc atcaggggcg
     8821 gcacttttcc tccatgcgcc tcgatgaact cagccgcttc cagcactgac cagttatttt
     8881 cacgctttcg ttcgtatgcc agcttaacaa tgccagcttg ccccatagat aaagcgtgct
     8941 tttccgcctc ccggctttct tttcgatagt tattccggat gctgtaaatg gtgttgatca
     9001 ggctgcttat ctgcgcggaa cagctgttta gcatgctcgc gatacggtat tcaggcggag
     9061 taccttcatc atcgtctttt tgctgatcgc gcatttcctg caccaggcga atacacgtat
     9121 ccctggcgtt ctccagcata aggagatgag aaagagactt ttccagaaga gtggtttcca
     9181 gaacatcggc cccggaccga cgcaacatag cgcgcgcggc cttccgcgct tcaacgttat
     9241 ctatcaggta atcgccaact tcgaactcaa agcgttcacc atcatcatcc agggtgtcgc
     9301 gttccaggcg atcacgtaag gtccggtggg cgcgggtgat cacgtcatga tcatctgaac
     9361 gatcatttat gcgcttattt tggcgcttcg cattctcgac tgcggcactg acaacggcat
     9421 taactctttg tttttccgct atttcagccg caatgtgatc acctgcatgt tgatcattag
     9481 agtgatcaat gatcatgctt tttagtggct tcctgactgg cttatttggc ttgcggctgt
     9541 ccgtagtcct ggtgtcttct ttgaaggcac ggagataacg acgtgcggta ttagggttaa
     9601 gattaaactc ggcggcatac tgtgcgatgg tgtaaccacc atctcgcgcc aggcgagcaa
     9661 aattcttctt gtgatcgtcc caggtcactt atgcttcctt tcgagatccc aacaatattc
     9721 ctactcaatg aacaaatgac tactcgtaga atcggttaac acaccagatt cagatcctta
     9781 ccctctgaat cctgccggta taccccattg ttcgttatct ttatttttgg ctaaaaccgc
     9841 attaagagct tcgtttaccg tcatgcaatg cggtaggtta tcgaagtttg atatcccgcc
     9901 aatatcaggc gaacgcttgt ttttcaggta agcatatttc cgcgcagccg cctctacttt
     9961 ctgcttgaac tcatgttttt gagtgcgttt tttggataac cgcagattgt cagcctttgc
    10021 ttttgcctta gcgatccatg aagtcaattt tttgaggctg gttgttccgg caccgccgga
    10081 aactgatctt tttgtttttt taacttgtga cttcttattc tttattgcca cgtcatcctg
    10141 acagggggag ggggtatcat tttgacatgg gggtgtggat aaaaaattaa ataaagccaa
    10201 tgtcttagcg agaacagctt taaccttggt tgccgctgac gaaatcttta atttgctttc
    10261 tatcagcgca tttttggctt gttgtgcgaa ggccaaaaag gatggtgtaa accggtacag
    10321 gttagcgcga cgttcacggt gatcgccgat aacaatctct acagacagaa tacctttgtt
    10381 tacagcttca cggaatgcac gaacgacggt tgattggcta taaccagttt ctgccgcgat
    10441 caggcggtga ggcttgtgaa tgaagtattc actggttgtt gccgcgagat ttgcacattg
    10501 cgacaggata tgcccggcgc tacgggatag accggagtgt gttacaaagc aggccaattc
    10561 atagccagaa aaagtaaaat cgctttaaga tccccgagtt ctcttaaacc aaggtttagg
    10621 attgaaatga tgacgccgga aacttcttat aaagcgtgga aacagccaca tcatagatga
    10681 ttgcaacctg cttacggggg atgcccttct ccagcaatcg ccgcatttgc tgccatgttt
    10741 cttcttggta tttaggccga cgcccaccta tacgaccttc tgcgcgagct gcatcaagtc
    10801 cagcgcgtgt acgttcaacg ataagctcac gttccatttc tgccagcgcc cccattacgt
    10861 gaaagaaaaa gcgccccatt ggtgtactgg tgtcgatgga gtcagtgaga ctccggaagt
    10921 taatgcctct gtcacgcagc tcttccacca gcacaactaa gtgacgcatg ctgcgcccaa
    10981 gacggtctaa cttccatacg accagggtat cacctctgga aagttacgga gtaccttttt
    11041 taacccaggg cgctcagcct ttttgccgct cgccttgtcc tcaaaaatta gctcacatcc
    11101 tgcgctttca agagcgtttc gttgtaaagc agtgttttgt tcatttgttg atacgcgtac
    11161 atagcctatt agagatccta taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac
    11221 tctagtagag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
    11281 gagcctttcg ttttatttga tgcctggaga tccttattgt gagtctggct ggttagtggc
    11341 catgagatat tcgattgtgt cagtgagatc atccaggtcg tcttgggtga tgcggtactc
    11401 ctgattggat atctttgagt agtgttcagc aatggcgcgg gcagcgtcgg tttcggcggg
    11461 gtctacagat aaagcgttag agcaatgtct aacgtcgtcg atggttggtg gaatgaaagc
    11521 cataattatg cctcactgta agatccgggt atggagaaac agtagagagt tgcgataaaa
    11581 agcgtcaggt aagatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga
    11641 cgccgtgcaa ataatcaatg tggacttttc tgccgtgatt atagacactt ttgttacgcg
    11701 tttttgtcat ggctttggtc ccgctttgtt acagaatgct tttaataagc ggggttaccg
    11761 gtttggttag cgagaagagc cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg
    11821 gacaattggt ttcttctctg aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg
    11881 atcccctgct gccgggatac tcgtttaatg cccatctggt ggcgggttta acgccgattg
    11941 aggccaacgg ttatctcgat ttttttatcg accgaccgct gggaatgaaa ggttatattc
    12001 tcaatctcac cattcgcggt cagggggtgg tgaaaaatca gggacgagaa tttgtttgcc
    12061 gaccgggtga tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg
    12121 aggctcgcga atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat
    12181 ggcttaactg gccgtcaata tttgccaata cggggttctt tcgcccggat gaagcgcacc
    12241 agccgcattt cagcgacctg tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct
    12301 attcggagct gctggcgata aatctgcttg agcaattgtt actgcggcgc atggaagcga
    12361 ttaacgagtc gctccatcca ccgatggata atcgggtacg cgaggcttgt cagtacatca
    12421 gcgatcacct ggcagacagc aattttgata tcgccagcgt cgcacagcat gtttgcttgt
    12481 cgccgtcgcg tctgtcacat cttttccgcc agcagttagg gattagcgtc ttaagctggc
    12541 gcgaggacca acgtatcagc caggcgaagc tgcttttgag caccacccgg atgcctatcg
    12601 ccaccgtcgg tcgcaatgtt ggttttgacg atcaactcta tttctcgcgg gtatttaaaa
    12661 aatgcaccgg ggccagcccg agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg
    12721 tagccgtcaa gttgtcataa ttggtaacga atcagacaat tgacggctcg atggagtagc
    12781 atagagatct cat
//
