LOCUS       sequence-117912-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S. solfataricus PCNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Hirakawa H, Nagamune T
  TITLE     Molecular assembly of P450 with ferredoxin and ferredoxin reductase 
            by fusion to PCNA.
  JOURNAL   Chembiochem. 2010 Jul 26;11(11):1517-20. doi: 
            10.1002/cbic.201000226.
  PUBMED    20607777
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cbic"; 
            journalName: "Chembiochem"; date: "2010-07-26- 26"; volume: "11"; 
            issue: "11"; pages: "1517-20"
COMMENT     Sequence Label: pHSG-PCNA2
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     116..138
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     130..147
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     131..147
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(954..970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(954..970)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(967..989)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    978..994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
       61 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      121 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcagca tatgatgaaa
      181 gctaaggtaa ttgacgctgt ctcattctct tatatattaa gaactgtggg tgacttcttg
      241 agtgaagcca actttattgt tactaaggag ggtataagag ttagcggaat tgatccttca
      301 agagtagttt ttctagatat atttctccca tcgagttact ttgagggatt tgaagttagt
      361 caagagaagg aaataatagg gtttaagtta gaggatgtaa atgatatact aaaacgtgtt
      421 ttaaaggacg atacgttaat actatcctcg aacgaatcta aattaacact tacttttgat
      481 ggagaattca ctagatcttt cgagcttcca ttaattcaag ttgaatctac acagccgcct
      541 tcagtcaatt tagagtttcc atttaaggct caactactca ctataacttt tgcggatata
      601 attgacgagt tatctgattt aggtgaagtt ttgaatatac actccaagga gaataaactt
      661 tattttgaag ttattggaga cctctccact gcaaaagtag agttgtcaac ggataacggt
      721 acgttattag aagcttctgg cgcagatgtc agtagcagtt atgggatgga atacgttgcg
      781 aatacaacta aaatgcgtcg cgcttccgat tcaatggaat tgtattttgg ttctcaaatt
      841 cctttgaagc ttcgatttaa attaccgcag gaaggctatg gggactttta cattgcacca
      901 agggcagact gaagagcgga tccccgggta ccgagctcga attcgtaatc atggtcatag
      961 ctgtttcctg tgtgaaattg ttatccgctc acaat
//
