LOCUS       sequence-117914-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS2 carrying mutation to introduce
            HindIII site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS2_HindIII
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..113)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcgggtttaa cgggccctct agactcgagg cacactggat cagttatcta tgcggccgcg
       61 gatcaggtac gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac
      121 cgcgggccct ctagacttga gcggccgcca ctgtgctgga tatcaaccac tttgtacaag
      181 aaagctgggt gtcaacagaa ctgacggtac ttccatcctt gctgggtggt gtcagaggaa
      241 gggtaccagc gcacctgagc gtggagggcg gcggcagcgg cagtggcagt tgggtaggtg
      301 aacgcagcat gggagatggc tggggtcagc tctgtggtgt acaaagggta gggggcccag
      361 gcctctgggg atgcagggat cagagcagcc cccatcaggt cataggggtc ccgtgcgatg
      421 aagtgtgctc ctagggcggg gtgcacgttg ctgggatttg gagttgccat tagcttgctc
      481 ttggccatct tggtgttggc tttggcaaac tctagcctca gagtctgtgg attttcggga
      541 tcaaagcgaa taccgttcag cgcattcttg gccgcttctg ctcctgcacg gctgtcaaag
      601 atcacaaaac caacaggctg tcttgcagtg agcttgatca gggacccttc ataccccttg
      661 aacggccgga agagcaagta gagttctctg ggtttaatgt ccacagggag gccgctgaca
      721 aacagtgtcc ggacctcct
//
