LOCUS       sequence-117924-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Liu JJ, Lindquist S
  TITLE     Oligopeptide-repeat expansions modulate 'protein-only' inheritance 
            in yeast.
  JOURNAL   Nature 1999 Aug 5;400(6744):573-6.
  PUBMED    10448860
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "1999-08-5"; volume: "400(6744)"; pages: "573-6"
COMMENT     Sequence Label: pJLI-SUP35 (delta)2-5
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(475..498)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 attgtaatac gactcactat agggcgaatt ggagctccac cgcggtgaaa agagtcagtg
       61 agacgacgac ttcaggatct ttgggtttca ggatatgtgg catgaaaata cagaaaaatc
      121 cctctgtgct gaatcagctt tcgctcgaat attacgaaga agaagcagac agtgattata
      181 tctttataaa caaattgtat ggtcgttcaa gaaccgatca aaatgtttca gatgcaattg
      241 aactttattt taacaatcct catctgtcgg atgcgagaaa gcatcaactg aagaaaacat
      301 ttttgaaaag attgcagttg ttttataata ctatgctaga agaagaagtt agaatgatat
      361 caagtagtct tttgtttatt tacgaaggag acccggagcg atgggaatta ctaaatgatg
      421 ttgacaaact tatgcgagat gattttatag acgatgatga cgacgatgat gataatgatg
      481 atgatgatga tgatgatgcc gagggaagca gcgaaggacc aaaggacaaa aaaacaactg
      541 gttctttgag ttccatgtca ctaatagatt ttgcacattc tgaaataacg ccggggaagg
      601 gttatgatga aaacgtgatt gaaggagttg aaaccttgct agatattttt atgaaattct
      661 agatatattg agaggtgaag tttaccttgt ttatggtata tggtacaaaa agaactaaac
      721 taattatacg tctatatata tatatatata tataacagct ttattaaacc ttgtttttta
      781 atatagaaga aaatgcttta tgatcggtat tattgtgttt gcatttactt atgtttgcaa
      841 gaaatggatc cttactcggc aattttaaca attttaccaa ttgctattgt gg
//
