LOCUS       sequence-117925-         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Atasoy D, Aponte Y, Su HH, Sternson SM
  TITLE     A FLEX switch targets Channelrhodopsin-2 to multiple cell types for 
            imaging and long-range circuit mapping.
  JOURNAL   J Neurosci. 2008 Jul 9. 28(28):7025-30.
  PUBMED    18614669
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Jul 9. 28(28):7025-30."
COMMENT     Sequence Label: AAV-FLEX-rev-ChR2(H134R)-mCherry
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    64..97
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    168..201
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      219..228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcgaac ctcttcgagg
       61 gacataactt cgtataatgt atgctatacg aagttatggg tcgatggtga gatctggact
      121 agagggtcga tggtgatgct tggatcaaaa taggaagaat cccaatgata acttcgtata
      181 aagtatccta tacgaagtta tactagaatt cggtaccggc caccatggac tatggcggcg
      241 ctttgtctgc cgtcggacgc gaacttttgt tcgttactaa tcctgtggtg gtgaacgggt
      301 ccgtcctggt ccctgaggat caatgttact gtgccggatg gattgaatct cgcggcacga
      361 acggcgctca gaccgcgtca aatgtcctgc agtggcttgc agcaggattc agcattttgc
      421 tgctgatgtt ctatgcctac caaacctgga aatctacatg cggctgggag gagatctatg
      481 tgtgcgccat tgaaatggtt aaggtgattc tcgagttctt ttttgagttt aagaatccct
      541 ctatgctcta ccttgccaca ggacaccggg tgcagtggct gcgctatgca gagtggctgc
      601 tcacttgtcc
//
