LOCUS       sequence-117931-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Badura A, Sun XR, Giovannucci A, Lynch LA, Wang SS
  TITLE     Fast calcium sensor proteins for monitoring neural activity.
  JOURNAL   Neurophotonics. 2014 Oct;1(2):025008.
  PUBMED    25558464
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neurophotonics."; date: "2014-10"; volume: "1(2)"; pages: "025008"
COMMENT     Sequence Label: Fast-GCaMP6f-RS06
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             149..166
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             170..202
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(206..214,215..229)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      236..245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             257..313
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     526..547
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(653..674)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(914..933)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag gatctcgatc ccgcgaaatt
       61 aatacgactc actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgggttctca tcatcatcat catcatggta tggctagcat
      181 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac
      241 catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg
      301 tcggctgagc tcactcgaga acgtctatat caaggccgac aagcagaaga acggcatcaa
      361 ggcgaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta
      421 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      481 cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      541 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg
      601 agggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      661 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc
      721 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      781 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      841 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac
      901 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      961 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1021 ggggcacaag ctggagtaca acctg
//
