LOCUS       sequence-117935-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSG5 containing N terminally HA tagged mouse SADBs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera
            AC
  TITLE     SADB phosphorylation of gamma-tubulin regulates centrosome 
            duplication.
  JOURNAL   Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 
            2009 Aug 2.
  PUBMED    19648910
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1921"; 
            journalName: "Nat Cell Biol"; date: "2009-09"; volume: "11"; issue: 
            "9"; pages: "1081-92"
COMMENT     Sequence Label: pSG5 N-HA SADBs
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     89..108
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     136..155
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        136..154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             174..200
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
       61 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
      121 tttggcaaag aattgtaata cgactcacta tagggcgaat tgatatcacc atgtacccgt
      181 acgatgtccc ggattacgcg aattcggacc agggcaccat gtcgtccggg tccaaggaag
      241 gtggcggggg ctcccccgcc taccacctcc cacgcccaca cccacaccca ccccagcacg
      301 cccaatatgt gggcccctat cgcctggaga agacgctggg caaaggacag acaggtctag
      361 ttaaacttgg agtccactgc atcacgggtc agaaggtcgc tgtcaagatc gtgaacaggg
      421 agaagctgtc ggaatcggtg ctgatgaagg tggagaggga aatcgccatc ctgaagctca
      481 ttgaacaccc gcacgtgctc aagctccacg acgtctacga gaacaagaaa tatttatact
      541 tggttcttga gcacgtttct ggtggtgagc tgttcgacta cctggtaaaa aaagggagac
      601 tgacacccaa ggaggcccgg aagttcttcc gccagatcgt gtcagcactg gacttctgcc
      661 atagctactc catctgtcac agagacttga agccagagaa cctgctgttg gatgagaaaa
      721 acaacatccg catcgcagac tttggtatgg cgtccctgca agtgggggac agcctcctgg
      781 agaccagctg cgggtccccc cattacgcat gtccagaggt gatcaagggg gaaaagtatg
      841 atggccgccg ggcagacatg tggagctgtg gagtcatcct atttgccctg cttgtggggg
      901 cactgccctt cgatgacgac aacctgcgcc agctactgga gaaggtgaaa cgtggggtct
      961 tccacatgcc tcacttcatc cctccagact gccagagcct cctgagaggg atgattgaag
     1021 tggagcccga gaaaaggctc agtctgga
//
