LOCUS       sequence-117938-        8338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  controls the expression of S. pyogenes dCas9 from a Tc‐inducible 
            PTet promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 8338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PTet-dCas9
FEATURES             Location/Qualifiers
     source          1..8338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(308..852)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(388..407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(941..1756)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(941..1756)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1664..1683
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1748..1767
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1934..1952
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1959..1977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2000..2011
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2018..2044,2045..2047,2048..4534,4535..4537,
                     4538..6124)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      6148..6219
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6235..6262
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             6355..7437
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6374..6393)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             7456..8079
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(7529..7548)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(8262..8281)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttcctcgc tcactgactc gctgcacgag gcagacctca gcgctagcgg agtgtatact
       61 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      121 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      181 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      241 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      301 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      361 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
      421 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      481 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
      541 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
      601 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
      661 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
      721 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
      781 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
      841 aaacgatctc aagaagatca tcttattaag gggtctgacg ctcagtggaa cgaaaaatca
      901 atctaaagta tatatgagta aacttggtct gacagttacc ttagaaaaac tcatcgagca
      961 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1021 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     1081 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     1141 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     1201 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     1261 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     1321 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     1381 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     1441 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     1501 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     1561 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     1621 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     1681 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctggagcaa gacgtttccc
     1741 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     1801 ttcatgatga tatattttta tcttgtgcaa tgtacatcag agattttgag acacaaccaa
     1861 ttattgaagg cctccctaac ggggggcctt tttttgtttc tggtctcccg ctttactcca
     1921 ccgttggctt ttttccctat cagtgataga gattgacatc cctatcagtg atagagataa
     1981 tgagcacttc aaaagatcta aagaggagaa aggatctatg gataagaaat actcaatagg
     2041 cttagctatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc
     2101 gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat
     2161 aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc
     2221 tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa
     2281 tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga
     2341 agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta
     2401 tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa
     2461 agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt
     2521 tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt
     2581 ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc
     2641 taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca
     2701 gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt
     2761 gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa
     2821 agatacttac gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga
     2881 tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt
     2941 aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca
     3001 tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa
     3061 agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag
     3121 ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga
     3181 attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg
     3241 ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga
     3301 cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat
     3361 tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa
     3421 gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc
     3481 tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact
     3541 accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa
     3601 atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat
     3661 tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta
     3721 tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa
     3781 tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga
     3841 taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga
     3901 tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat
     3961 gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa
     4021 tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt
     4081 tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat
     4141 tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc
     4201 tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt
     4261 caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca
     4321 gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat
     4381 caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa
     4441 tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt
     4501 agatattaat cgtttaagtg attatgatgt cgatgccatt gttccacaaa gtttccttaa
     4561 agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga
     4621 taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa
     4681 cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt
     4741 gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac
     4801 taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa
     4861 acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa
     4921 agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta
     4981 tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt
     5041 tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga
     5101 aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac
     5161 agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga
     5221 aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc
     5281 catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga
     5341 gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc
     5401 aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa
     5461 ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat
     5521 tatggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa
     5581 ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa
     5641 cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct
     5701 gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag
     5761 tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga
     5821 gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga
     5881 taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa
     5941 tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga
     6001 tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat
     6061 ccatcaatcc atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga
     6121 ctaactcgag taaggatctc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     6181 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     6241 ccttcgggtg ggcctttctg cgtttataaa tggcggcgcg ccatcgaatg gcgcaaaacc
     6301 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatatgaaa
     6361 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
     6421 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
     6481 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
     6541 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
     6601 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
     6661 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
     6721 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
     6781 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
     6841 catgaggacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
     6901 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
     6961 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
     7021 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
     7081 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
     7141 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagatag ctcatgttat
     7201 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     7261 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gccagtctca
     7321 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7381 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgataa
     7441 tccaggagga aaaaaatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
     7501 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
     7561 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
     7621 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
     7681 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
     7741 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
     7801 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
     7861 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
     7921 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
     7981 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
     8041 ggattagaaa aacaacttaa atgtgaaagt gggtcctaat aattggtaac gaatcagaca
     8101 attgacggct cgagggagta gcatagggtt tgcagaatcc ctgcttcgtc catttgacag
     8161 gcacattatg catcgatgat aagctgtcaa acatgagcag atcctctacg ccggacgcat
     8221 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     8281 cgatggggaa gatcgggctc gccacttcgg gctcatgagc aaatatttta tctgaggt
//
