LOCUS       sequence-117941-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA targeting human ARF4a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Volpicelli-Daley LA, Li Y, Zhang CJ, Kahn RA
  TITLE     Isoform-selective effects of the depletion of ADP-ribosylation 
            factors 1-5 on membrane traffic.
  JOURNAL   Mol Biol Cell. 2005 Oct;16(10):4495-508. Epub 2005 Jul 19.
  PUBMED    16030262
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2005-10"; volume: "16(10)"; pages: "4495-508. Epub 
            2005 Jul 19"
COMMENT     Sequence Label: pSUPER-ARF4a
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(111..127)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(156..174)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(195..211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(195..211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(208..230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(243..249,250..267,268..273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(426..443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(677..696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttggatttg ggatcttata agttctgtat gagaccacag atcccctctg gtagatgaat
       61 tgagattcaa gagatctcaa ttcatctacc agatttttgg aaaagcttat cgataccgtc
      121 gacctcgagg gggggcccgg taccagcttt tgttcccttt agtgagggtt aatttcgagc
      181 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      241 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      301 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      361 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      481 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      541 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      601 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      661 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      721 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      781 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      841 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      901 cgtcttgagt cc
//
