LOCUS       sequence-117943-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Liu JJ, Lindquist S
  TITLE     Oligopeptide-repeat expansions modulate 'protein-only' inheritance 
            in yeast.
  JOURNAL   Nature 1999 Aug 5;400(6744):573-6.
  PUBMED    10448860
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "1999-08-5"; volume: "400(6744)"; pages: "573-6"
COMMENT     Sequence Label: pJLI-SUP35 R2E2
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..751
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYKG"
     primer_bind     complement(62..90)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     685..705
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcat ggctagcaaa ggagaagaac tcttcactgg
       61 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      121 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      181 tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg gtgttcagtg
      241 cttttcaaga tacccggatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
      301 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      361 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      421 taaagaagat ggaaacattc ttgggcacaa attggaatac aactataact cacacaatgt
      481 atacatcatg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttagacacaa
      541 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      601 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      661 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
      721 acatggcatg gatgaactat acaaaggatg agagctcatc gttaacaact tcgtcatcca
      781 cttcttcttc ctgttccttg atcaaggcat cagcactggt aacattggca ttgttggtat
      841 tatgtgttga ttcagagatt ttaaggtctt ctacctttgg aagttcc
//
