LOCUS       sequence-117945-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Liu JJ, Lindquist S
  TITLE     Oligopeptide-repeat expansions modulate 'protein-only' inheritance 
            in yeast.
  JOURNAL   Nature 1999 Aug 5;400(6744):573-6.
  PUBMED    10448860
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "1999-08-5"; volume: "400(6744)"; pages: "573-6"
COMMENT     Sequence Label: pJLI-SUP35 R2E2
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     63..79
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     64..80
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        501..735
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
ORIGIN
        1 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggccccc
       61 cctcgaggtc gacggtatcg ataagcttga tatcgaattt cccattaccg acatttgggc
      121 ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
      181 catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
      241 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
      301 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
      361 aatttaactg tgataaacta ccgcattaaa gctaaaacac ttttgtatta tttttcctca
      421 tatatgtgta taggtttata cggatgattt aattattact tcaccaccct ttatttcagg
      481 ctgatatctt agccttgtta ctagttagaa aaagacattt ttgctgtcag tcactgtcaa
      541 gagattcttt tgctggcatt tcttctagaa gcaaaaagag cgatgcgtct tttccgctga
      601 accgttccag caaaaaagac taccaacgca atatggattg tcagaatcat ataaaagaga
      661 agcaaataac tccttgtctt gtatcaattg cattataata tcttcttgtt agtgcaatat
      721 catatagaag tcatcgaaat agatattaag aaaaacaaac tgtacaatca atcaatcaat
      781 caggatccat gtcggattca aaccaaggca acaatcagca aaactaccag caatacagcc
      841 agaacggtaa ccaacaacaa ggtaacaaca ga
//
