LOCUS       sequence-117948-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-regulated mammalian expression vector for expressing 
            shRNA against human SADB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera
            AC
  TITLE     SADB phosphorylation of gamma-tubulin regulates centrosome 
            duplication.
  JOURNAL   Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 
            2009 Aug 2.
  PUBMED    19648910
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1921"; 
            journalName: "Nat Cell Biol"; date: "2009-09"; volume: "11"; issue: 
            "9"; pages: "1081-92"
COMMENT     Sequence Label: pTER SADB shRNA Human
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     153..172
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    218..236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(408..425)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    414..638
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      684..1042
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(771..790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     981..1002
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccgggctgca ggaaattcga acgctgacgt catcaacccg ctccaaggaa tcgcggccca
       61 gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg
      121 acaggggagt ggcgccctgc aatatttgca tgtcgctatg tgttctggga aatcaccata
      181 aacgtgaaat gtctttggat ttgggaatct tataagttcc ctatcagtga tagagatccc
      241 gctccacgac gtctacgagt tcaagagact cgtagacgtc gtggagcttt ttggaaaagc
      301 ttggtaccga gctcggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca
      361 gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
      421 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      481 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      541 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      601 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      661 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      841 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      901 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      961 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     1021 ctcggtctat tcttttgatt ta
//
